Composite

Part:BBa_K4773019:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-02)


Lac Promoter+RBS+HAO+RBS+HmpA+Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1586
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Term (BBa_B0015) was chosen because it is a double terminator, which can effectively terminate transcription and protect mRNA from degradation.RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts.


Source

HAO: From the genomic sequence of Nitrosomonas europaea. HmpA: From the genomic sequence of Escherichia coli.

References